igfbp-6 protein (PeproTech)
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igfbp-6 protein
Igfbp 6 Protein, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igfbp-6+protein/igfbp+6+protein/pmc09322660-56-27-13
Average 90 stars, based on 1 article reviews
Igfbp 6 Protein, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igfbp-6+protein/igfbp+6+protein/pmc09322660-56-27-13
Average 90 stars, based on 1 article reviews
igfbp-6 protein - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Insulin-Like Growth Factor Binding Protein (IGFBP-6) as a Novel Regulator of Inflammatory Response in Cystic Fibrosis Airway Cells"
Article Title: Insulin-Like Growth Factor Binding Protein (IGFBP-6) as a Novel Regulator of Inflammatory Response in Cystic Fibrosis Airway Cells
Journal: Frontiers in Molecular Biosciences
doi: 10.3389/fmolb.2022.905468
Figure Legend Snippet: IGFBP-6 shows higher expression in F508del-CFTR CFBE cells and is further increased after LPS treatment. (A) Total RNA was extracted and qRT-PCR was performed in order to quantify IGFBP-6 mRNA normalized to GADPH as housekeeping gene. Data represent the mean ± SEM ( n = 3). Statistical significance tested using paired two-tailed t -test. (B) Immunoblots of basal expression of IGFBP-6 in Wt and F508del-CFTR CFBE cells. (C) Data represent the mean ± SEM of the ratio IGFBP-6/Calnexin ( n = 3). Statistical significance tested using paired two-tailed t -test. (D,E) Cells were treated with 1 μg/ml LPS for 4 h. Total RNA was extracted and qRT-PCR was performed in order to quantify IGFBP-6 mRNA s normalized first to GADPH as housekeeping gene and then to control untreated cells. Data represent the mean ± SEM ( n = 4). Statistical significance tested using two-way ANOVA with Tukey’s multiple comparisons test (F) Immunoblots of IGFBP-6 following treatment with 1 μg/ml LPS for 24 h. (G) Data represent the mean ± SEM of the ratio IGFBP-6/Calnexin normalized to Calnexin control ( n = 3–4). * p < 0.05; ** p < 0.01; **** p < 0.0001.
Techniques Used: Expressing, Quantitative RT-PCR, Two Tailed Test, Western Blot
Figure Legend Snippet: IGFBP-6 is reduced by Dimethyl fumarate. (A) Cells were treated with 0.1% DMSO, 1 μg/ml LPS ± 50 µM DMF or (B) PBS, 30 ng/ml IL-1β + 30 ng/ml TNFα ± 50 µM DMF for 4 h. Total RNA was extracted and qRT-PCR was performed in order to quantify IGFBP mRNA normalized to GADPH as housekeeping gene and then to control untreated cells. Data represent the mean ± SEM ( n = 4). (C) IGFBP-6 secretion after stimulation with 0.1% DMSO, 1 μg/ml LPS, 30 ng/ml IL-1β + 30 ng/ml TNFα ± 50 µM DMF for 24 h. Data represent the mean ± SEM ( n = 3). * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Statistical significance tested using two-way ANOVA with Tukey’s multiple comparisons test to the control (Wt or F508del) for each cell line.
Techniques Used: Quantitative RT-PCR
Figure Legend Snippet: Anti-IGFBP-6 antibodies increased pro-inflammatory cytokines expression. (A) F508del-CFTR CFBE cells were treated with PBS or 0.2–200 ng/ml IGFBP-6 for 4 h. Statistical significance tested using two-way ANOVA with Tukey’s multiple comparisons test. (B) Cells were treated with 1 μg/ml LPS ± an antibody directed against IGFBP-6 (1 μg/ml) for 4 h. Total RNA was extracted and qRT-PCR was performed in order to quantify IL-1β, IL-6 and TNF-α mRNAs normalized to GADPH as housekeeping gene and then to control untreated cells. Data represent the mean ± SEM ( n = 3). Statistical significance tested using paired two-tailed t -test.
Techniques Used: Expressing, Quantitative RT-PCR, Two Tailed Test
Figure Legend Snippet: IGFBP-6 shows higher expression in F508del / F508del nasal epithelial cells from three CF donors and reduces pro-inflammatory cytokines expression. (A) Total RNA was extracted from 2 patients homozygous for F508del mutation and 2 non-CF donors. qRT-PCR was performed in order to quantify IGFBP-6 mRNA normalized to GADPH as housekeeping gene. Data represent the mean ± SEM ( n = 4). Statistical significance tested using paired two-tailed t -test (B) Cells were treated with 0.1% DMSO, 1 μg/ml LPS, 30 ng/ml IL-1β + 30 ng/ml TNFα ± 50 µM DMF for 4 h. Total RNA was extracted and qRT-PCR was performed in order to quantify IGFBP mRNA normalized to control untreated cells. Data represent the mean ± SEM ( n = 4). (C) Cells were treated with 200 ng/ml IGFBP-6 for 4 h. Total RNA was extracted and qRT-PCR was performed in order to quantify IL-1β, IL-6 and TNFα mRNAs normalized to control untreated cells. Data represent the mean ± SEM ( n = 4). * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Statistical significance tested using two-way ANOVA with Tukey’s multiple comparisons test to the control for (Wt or F508del) each cell line.
Techniques Used: Expressing, Mutagenesis, Quantitative RT-PCR, Two Tailed Test
Related Articles
Expressing:Article Title: Insulin-Like Growth Factor Binding Protein (IGFBP-6) as a Novel Regulator of Inflammatory Response in Cystic Fibrosis Airway Cells Article Snippet: Cells were treated for 24 h with 0.1% DMSO or 200 ng/ml Article Title: Insulin-Like Growth Factor Binding Protein 6 Is Secreted in Extracellular Vesicles upon Hyperthermia and Oxidative Stress in Dendritic Cells But Not in Monocytes. Article Snippet: Purified Quantitative RT-PCR:Article Title: Insulin-Like Growth Factor Binding Protein (IGFBP-6) as a Novel Regulator of Inflammatory Response in Cystic Fibrosis Airway Cells Article Snippet: Cells were treated for 24 h with 0.1% DMSO or 200 ng/ml Article Title: Insulin-Like Growth Factor Binding Protein 6 Is Secreted in Extracellular Vesicles upon Hyperthermia and Oxidative Stress in Dendritic Cells But Not in Monocytes. Article Snippet: Purified Two Tailed Test:Article Title: Insulin-Like Growth Factor Binding Protein (IGFBP-6) as a Novel Regulator of Inflammatory Response in Cystic Fibrosis Airway Cells Article Snippet: Cells were treated for 24 h with 0.1% DMSO or 200 ng/ml Article Title: Insulin-Like Growth Factor Binding Protein 6 Is Secreted in Extracellular Vesicles upon Hyperthermia and Oxidative Stress in Dendritic Cells But Not in Monocytes. Article Snippet: Purified Western Blot:Article Title: Insulin-Like Growth Factor Binding Protein (IGFBP-6) as a Novel Regulator of Inflammatory Response in Cystic Fibrosis Airway Cells Article Snippet: Cells were treated for 24 h with 0.1% DMSO or 200 ng/ml Article Title: Insulin-Like Growth Factor Binding Protein 6 Is Secreted in Extracellular Vesicles upon Hyperthermia and Oxidative Stress in Dendritic Cells But Not in Monocytes. Article Snippet: Purified Mutagenesis:Article Title: Insulin-Like Growth Factor Binding Protein (IGFBP-6) as a Novel Regulator of Inflammatory Response in Cystic Fibrosis Airway Cells Article Snippet: Cells were treated for 24 h with 0.1% DMSO or 200 ng/ml Article Title: Insulin-Like Growth Factor Binding Protein 6 Is Secreted in Extracellular Vesicles upon Hyperthermia and Oxidative Stress in Dendritic Cells But Not in Monocytes. Article Snippet: Purified Purification:Article Title: Insulin-Like Growth Factor Binding Protein (IGFBP-6) as a Novel Regulator of Inflammatory Response in Cystic Fibrosis Airway Cells Article Snippet: Cells were treated for 24 h with 0.1% DMSO or 200 ng/ml Article Title: Insulin-Like Growth Factor Binding Protein 6 Is Secreted in Extracellular Vesicles upon Hyperthermia and Oxidative Stress in Dendritic Cells But Not in Monocytes. Article Snippet: Purified Positive Control:Article Title: Insulin-Like Growth Factor Binding Protein (IGFBP-6) as a Novel Regulator of Inflammatory Response in Cystic Fibrosis Airway Cells Article Snippet: Cells were treated for 24 h with 0.1% DMSO or 200 ng/ml Article Title: Insulin-Like Growth Factor Binding Protein 6 Is Secreted in Extracellular Vesicles upon Hyperthermia and Oxidative Stress in Dendritic Cells But Not in Monocytes. Article Snippet: Purified |